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KMID : 0355519880120020135
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Korean Journal of Oral Biology
1988 Volume.12 No. 2 p.135 ~ p.144
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EFFECTS OF CYCLIC NUCLEOTIDE DERIVATIVES ON ACTINAR CELL MEMBRANE POTENTIAL AND RESISTANCE IN THE RAT SUBMAXILLARY GLAND
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Abstract
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The purpose of this experiment was to clarify the effects of exogenous dibutyryl cyclic GMF´(dB cGMP), 8-bromo cycle GMP(8-cB GMP) and dibutyryl cyclic AMP(dB CAMP) on the :membrane potential and input resistance of salivary acinar cells and the role of calcium on the effects of cyclic nucleotide derivatives in isolated submaxillary gland of rats.
Segments of submaxillary gland were isolated and superfused with normal Krebs-Henseleit solution which contained theophylline(IOmM). Calcium concentration in Krebs-Henseleit solution was 2.5mM and ouabain was added to the superfusion solution to give a final concentration of 10-´M Cyclic nucleotide derivatives(10-´M) were added directly to the tissue bath at the rate of 0.57m1/min by constant infusion pump.
Membrane potential and input resistance were measured with glass microelectrode filled with 3M KCI, having a tip resistance of 10-30MQ.
1. The mean resting membrane potential and input resistance of submaxillary gland cells were -19.4 mV and 16.OM Q, resispectively.
2. d.B cGMP(10-´M) evoked hyperpolarization by 7.8mV and membrane input resistance was not reduced.
3. In Krebs -Henseleit solution containing ouabain( 10-4M), dB cGMP evoked hyperpolarization by 6.8mV and it took more time to maximum hyperpolarization than ouabain free group.
4. In Ca free Krebs-Henseleit solution containing EGTA(10-´M), dB cGMP evoked hyperpolarization by 3.7mV and membrane input resistance was not reduced.
5. 8-cB GMP(10-´M) and dB cAMP(10-4 M) did not show any change in membrane potential and input resistance.
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KEYWORD
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